n-Butanol Extract of Lotus Seeds Exerts Antiobesity Effects in 3T3-L1 Preadipocytes and High-Fat Diet-Fed Mice via Activating Adenosine Monophosphate-Activated Protein Kinase.
Zhenyu WangJiamiao HuSiti Sarah HamzahShenghan GeYilin LinBaodong ZhengShaoxiao ZengShaoling LinPublished in: Journal of agricultural and food chemistry (2019)
In this study, the antiobesity effects of n-butanol extract of lotus seeds (LBE) were evaluated in cultured 3T3-L1 preadipocytes and in high-fat diet (HFD)-fed mice. LBE decreased lipid contents in mature 3T3-L1 cells without obvious cytotoxicity. Meanwhile, LBE supplementation also led to weight loss and improved plasma lipid profiles in HFD-fed mice. Furthermore, LBE could activate AMP-activated protein kinase (AMPK) accompanied by down-regulation of lipogenesis related genes (PPARγ, aP2, LPL, C/EBPα, FAS, SREBP-1c) and up-regulation of lipolysis genes (adiponectin and PPARα) in vitro and in vivo. Collectively, our data demonstrated LBE possesses antiadipogenic and antilipogenic activities which are, at least partially, mediated by the activation of AMPK signaling pathways.
Keyphrases
- high fat diet
- protein kinase
- insulin resistance
- high fat diet induced
- adipose tissue
- skeletal muscle
- metabolic syndrome
- weight loss
- signaling pathway
- induced apoptosis
- type diabetes
- oxidative stress
- fatty acid
- endothelial cells
- cell cycle arrest
- gene expression
- anti inflammatory
- transcription factor
- genome wide
- bariatric surgery
- epithelial mesenchymal transition
- pi k akt
- cell death
- machine learning
- gastric bypass
- artificial intelligence
- data analysis