Effect of Dietary Lipids on Endotoxemia Influences Postprandial Inflammatory Response.
Javier López-MorenoSonia García-CarpinteroRosa Jimenez-LucenaCarmen HaroOriol A Rangel-ZúñigaRuth Blanco-RojoElena M Yubero-SerranoFrancisco J TinahonesJavier Delgado-ListaPablo Pérez-MartínezHelen M RocheJosé López-MirandaAntonio CamargoPublished in: Journal of agricultural and food chemistry (2017)
Metabolic syndrome (MetS) results in postprandial metabolic alterations that predisposes one to a state of chronic low-grade inflammation and increased oxidative stress. We aimed to assess the effect of the consumption of the quantity and quality of dietary fat on fasting and postprandial plasma lipopolysaccharides (LPS). A subgroup of 75 subjects with metabolic syndrome was randomized to receive 1 of 4 diets: HSFA, rich in saturated fat; HMUFA, rich in monounsaturated fat; LFHCC n-3, low-fat, rich in complex carbohydrate diet supplemented with n-3 polyunsaturated fatty acids; LFHCC low-fat, rich in complex carbohydrate diet supplemented with placebo, for 12 weeks each. We administered a fat challenge reflecting the fatty acid composition of the diets at postintervention. We determined the plasma lipoproteins and glucose and gene expression in peripheral blood mononuclear cells (PBMC) and adipose tissue. LPS and LPS binding protein (LBP) plasma levels were determined by ELISA, at fasting and postprandial (4 h after a fat challenge) states. We observed a postprandial increase in LPS levels after the intake of the HSFA meal, whereas we did not find any postprandial changes after the intake of the other three diets. Moreover, we found a positive relationship between the LPS plasma levels and the gene expression of IkBa and MIF1 in PBMC. No statistically significant differences in the LBP plasma levels at fasting or postprandial states were observed. Our results suggest that the consumption of HSFA diet increases the intestinal absorption of LPS which, in turn, increases postprandial endotoxemia levels and the postprandial inflammatory response.
Keyphrases
- blood glucose
- inflammatory response
- adipose tissue
- fatty acid
- gene expression
- insulin resistance
- metabolic syndrome
- lps induced
- weight loss
- oxidative stress
- low grade
- lipopolysaccharide induced
- glycemic control
- anti inflammatory
- toll like receptor
- physical activity
- double blind
- randomized controlled trial
- dna damage
- type diabetes
- high grade
- weight gain
- heat shock protein
- induced apoptosis
- body mass index