Multiple genetically modified GTKO/hCD46/HLA-E/hβ2-mg porcine hearts are protected from complement activation and natural killer cell infiltration during ex vivo perfusion with human blood.
Jan-Michael AbichtRiccardo SfrisoBruno ReichartMatthias LänginKatja GahleGisella L Puga YungJörg Dieter SeebachRobert RiebenDavid AyaresEckhard WolfNikolai KlymiukAndrea BaehrAlexander KindTanja MayrAndreas BauerPublished in: Xenotransplantation (2018)
GTKO, hCD46, and HLA-E expression in porcine hearts reduced complement deposition, complement dependent injury, and myocardial NK cell infiltration during perfusion with human blood. This tested combination of genetic modifications may minimize damage from acute human-anti-pig rejection reactions and improve myocardial function after xenotransplantation.
Keyphrases
- endothelial cells
- induced pluripotent stem cells
- pluripotent stem cells
- nk cells
- left ventricular
- poor prognosis
- single cell
- heart failure
- magnetic resonance imaging
- gene expression
- intensive care unit
- genome wide
- respiratory failure
- long non coding rna
- copy number
- acute respiratory distress syndrome
- aortic dissection