Role of lncRNA LIPE-AS1 in adipogenesis.
Alyssa ThunenDeirdre La PlacaZhifang ZhangJohn E ShivelyPublished in: Adipocyte (2022)
Recent studies have identified long non-coding RNAs (lncRNAs) as potential regulators of adipogenesis. In this study, we have characterized a lncRNA, LIPE-AS1, that spans genes CEACAM1 to LIPE in man with conservation of genomic organization and tissue expression between mouse and man. Tissue-specific expression of isoforms of the murine lncRNA were found in liver and adipose tissue, one of which, designated mLas-V3, overlapped the Lipe gene encoding hormone-sensitive lipase in both mouse and man suggesting that it may have a functional role in adipose tissue. Knock down of expression of mLas-V3 using anti-sense oligos (ASOs) led to a significant decrease in the differentiation of the OP9 pre-adipocyte cell line through the down regulation of the major adipogenic transcription factors Pparg and Cebpa . Knock down of mLas-V3 induced apoptosis during the differentiation of OP9 cells as shown by expression of active caspase-3, a change in the localization of LIP/LAP isoforms of C/EBPβ, and expression of the cellular stress induced factors CHOP, p53, PUMA, and NOXA. We conclude that mLas-V3 may play a role in protecting against stress associated with adipogenesis, and its absence leads to apoptosis.
Keyphrases
- poor prognosis
- induced apoptosis
- long non coding rna
- adipose tissue
- endoplasmic reticulum stress
- stress induced
- oxidative stress
- signaling pathway
- transcription factor
- cell death
- cell cycle arrest
- insulin resistance
- binding protein
- high fat diet
- genome wide
- fatty acid
- long noncoding rna
- genome wide identification
- metabolic syndrome
- climate change
- dna binding