Trimethyltin(IV) Bearing 3-(4-Methyl-2-oxoquinolin-1(2H)-yl)propanoate Causes Lipid Peroxidation-Mediated Autophagic Cell Death in Human Melanoma A375 Cells.
Marijana P KasalovićDušan S DimićSanja JelačaDanijela Maksimović-IvanićSanja MijatovićBojana B ZmejkovskiSimon H F SchreinerTobias RüfferNebojša Đ PantelićGoran N KaluđerovićPublished in: Pharmaceuticals (Basel, Switzerland) (2024)
A novel trimethyltin(IV) complex ( Me 3 SnL ), derived from 3-(4-methyl-2-oxoquinolin-1(2H)-yl)propanoate ligand, has been synthesized and characterized by elemental microanalysis, UV/Vis spectrophotometry, FT-IR and multinuclear ( 1 H, 13 C and 119 Sn) NMR spectroscopies. Furthermore, the structure of the ligand precursor HL was solved using SC-XRD (single-crystal X-ray diffraction). The prediction of UV/Vis and NMR spectra by quantum-chemical methods was performed and compared to experimental findings. The protein binding affinity of Me 3 SnL towards BSA was determined by spectrofluorometric titration and subsequent molecular docking simulations. Me 3 SnL has been evaluated for its in vitro anticancer activity against three human cell lines, MCF-7 (breast adenocarcinoma), A375 (melanoma) and HCT116 (colorectal carcinoma), and three mouse tumor cell lines, 4T1 (breast carcinoma), B16 (melanoma) and CT26 (colon carcinoma), using MTT and CV assays. The strong inhibition of A375 cell proliferation, ROS/RNS upregulation and robust lipid peroxidation lead to autophagic cell death upon treatment with Me 3 SnL .
Keyphrases
- cell death
- cell cycle arrest
- molecular docking
- cell proliferation
- endothelial cells
- high resolution
- magnetic resonance
- induced pluripotent stem cells
- dual energy
- molecular dynamics
- squamous cell carcinoma
- induced apoptosis
- computed tomography
- skin cancer
- molecular dynamics simulations
- pi k akt
- fatty acid
- signaling pathway
- high throughput
- poor prognosis
- magnetic resonance imaging
- radiation therapy
- contrast enhanced
- oxidative stress
- breast cancer cells
- image quality
- single cell
- density functional theory
- endoplasmic reticulum stress
- mass spectrometry
- electron microscopy