Optimization of scleroglucan production by Sclerotium rolfsii by lowering pH during fermentation via oxalate metabolic pathway manipulation using CRISPR/Cas9.
Tianlong BaiTeng WangYan LiNa L GaoLixin ZhangWei-Hua ChenXiushan YinPublished in: Fungal biology and biotechnology (2021)
We established a platform for gene editing that could rapidly generate and select mutants to provide a new beneficial strain of S. rolfsii as a scleroglucan hyper-producer, which is expected to reduce the cost of controlling the optimum pH condition in the fermentation industry.