Genome-wide differential expression profiling of lncRNAs and mRNAs associated with early diabetic cardiomyopathy.
Tarun PantAnuradha DhanasekaranXiaowen BaiMing ZhaoEdward Benjamin ThorpJoseph M ForbessZeljko J BosnjakZhi-Dong GePublished in: Scientific reports (2019)
Diabetic cardiomyopathy is one of the main causes of heart failure and death in patients with diabetes. There are no effective approaches to preventing its development in the clinic. Long noncoding RNAs (lncRNA) are increasingly recognized as important molecular players in cardiovascular disease. Herein we investigated the profiling of cardiac lncRNA and mRNA expression in type 2 diabetic db/db mice with and without early diabetic cardiomyopathy. We found that db/db mice developed cardiac hypertrophy with normal cardiac function at 6 weeks of age but with a decreased diastolic function at 20 weeks of age. LncRNA and mRNA transcripts were remarkably different in 20-week-old db/db mouse hearts compared with both nondiabetic and diabetic controls. Overall 1479 lncRNA transcripts and 1109 mRNA transcripts were aberrantly expressed in 6- and 20-week-old db/db hearts compared with nondiabetic controls. The lncRNA-mRNA co-expression network analysis revealed that 5 deregulated lncRNAs having maximum connections with differentially expressed mRNAs were BC038927, G730013B05Rik, 2700054A10Rik, AK089884, and Daw1. Bioinformatics analysis revealed that these 5 lncRNAs are closely associated with membrane depolarization, action potential conduction, contraction of cardiac myocytes, and actin filament-based movement of cardiac cells. This study profiles differently expressed lncRNAs in type 2 mice with and without early diabetic cardiomyopathy and identifies BC038927, G730013B05Rik, 2700054A10Rik, AK089884, and Daw1 as the core lncRNA with high significance in diabetic cardiomyopathy.
Keyphrases
- heart failure
- network analysis
- type diabetes
- left ventricular
- wound healing
- long non coding rna
- genome wide
- cardiovascular disease
- long noncoding rna
- single cell
- binding protein
- poor prognosis
- dna methylation
- high fat diet induced
- primary care
- randomized controlled trial
- blood pressure
- induced apoptosis
- signaling pathway
- cardiovascular events
- clinical trial
- metabolic syndrome
- cell proliferation
- acute heart failure
- cell death
- copy number
- mass spectrometry
- wild type
- high resolution
- gestational age
- smooth muscle
- human health
- ejection fraction