Development of a Quorum Sensing-Mediated Bacterial Autolytic System in Escherichia coli for Automatic Release of Intracellular Products.
Xiaofei SongYifan ZhaoYixuan RenRuoyu LiuMengting ZhangZhikai ZhangQiu MengTingheng ZhuJianhua YinZhiliang YuPublished in: ACS synthetic biology (2024)
Escherichia coli , one of the most efficient expression hosts for recombinant proteins, is widely used in chemical, medical, food, and other industries. De novo engineering of gene regulation circuits and cell density-controlled E. coli cell lysis are promising directions for the release of intracellular bioproducts. Here, we developed an E. coli autolytic system, named the quorum sensing-mediated bacterial autolytic (QS-BA) system, by incorporating an acyl-homoserine lactone (AHL)-based YasI/YasR-type quorum sensing circuit from Pseudoalteromonas into E. coli cells. The results showed that the E. coli QS-BA system can release the intracellular bioproducts into the cell culture medium in terms of E. coli cell density, which offers an environmentally-friendly, economical, efficient, and flexible E. coli lysis platform for production of recombinant proteins. The QS-BA system has the potential to serve as an integrated system for the large-scale production of target products in E. coli for medical and industrial applications.
Keyphrases
- escherichia coli
- single cell
- cell therapy
- klebsiella pneumoniae
- healthcare
- biofilm formation
- induced apoptosis
- poor prognosis
- reactive oxygen species
- oxidative stress
- cell death
- wastewater treatment
- deep learning
- cell cycle arrest
- machine learning
- human health
- endoplasmic reticulum stress
- fatty acid
- climate change
- signaling pathway
- long non coding rna
- low cost