A transient CRISPR/Cas9 expression system for genome editing in Trypanosoma brucei.

Sebastian ShawSebastian KnüselSarah HoennerIsabel Roditi

Published in: BMC research notes (2020)

We describe a sequential transfection expression system that enables transient expression of the two proteins, followed by delivery of PCR products for gRNAs and repair templates. This procedure can be used for genome editing without the need for stable integration of the Cas9 and T7RNAP genes.

Keyphrases

genome editing
crispr cas
poor prognosis
binding protein
long non coding rna
gene expression