Primer-Engineered Transferase Enzyme for One-Pot and Amplified Detection of Cobalt Pollution and Peptide Remover Screening.
Yao RenLulu JinHongling ZengRosa BusquetsGuiping HeSha DengQiang HeMohammad Rizwan KhanRuijie DengYuanlong ChiPublished in: Journal of agricultural and food chemistry (2022)
Extensive consumption of cobalt in the chemical field such as for battery materials, alloy, pigments, and dyes has aggravated the pollution of cobalt both in food and the environment, and assays for its on-site monitoring are urgently demanded. Herein, we utilized enzyme dependence on metal cofactors to develop terminal transferase (TdT) as a recognition element, achieving a one-pot sensitive and specific assay for detecting cobalt pollution. We engineered a 3'-OH terminus primer to improve the discrimination capacity of TdT for Co 2+ from other bivalent cations. The TdT extension reaction amplified the recognition of Co 2+ and yielded a limit of detection of 0.99 μM for Co 2+ detection. Then, the TdT-based assay was designed to precisely detect cobalt in food and agricultural soil samples. By end-measurement of fluorescence using a microplate reader, the multiplexing assay enabled the rapid screening of the peptide remover for cobalt pollution. The TdT-based assay can be a promising tool for cobalt pollution monitoring and control.
Keyphrases
- heavy metals
- human health
- risk assessment
- reduced graphene oxide
- particulate matter
- high throughput
- health risk assessment
- loop mediated isothermal amplification
- metal organic framework
- carbon nanotubes
- climate change
- water quality
- real time pcr
- gold nanoparticles
- single molecule
- drinking water
- ionic liquid
- sensitive detection
- quantum dots