Anti-proliferative Effect of a Novel Anti-oxidative Peptide in Hanwoo Beef on Human Colorectal Carcinoma Cells.
Hye-Jin KimSe Ran YangAera JangPublished in: Korean journal for food science of animal resources (2018)
The present study aimed to characterise anti-oxidant peptides from water-soluble protein extracts of Hanwoo beef and evaluate their anti-proliferative effect on human colorectal carcinoma cells (HCT116). Antioxidant peptides were purified from the low-molecular-weight fraction (<3 kDa) of Hanwoo beef extract. Antioxidant activity of peptide fractions was determined using the oxygen radical absorbance capacity (ORAC) assay. Purified peptide (P3) displayed higher ORAC activity than the low-molecular-weight fraction (202.66 μM TE/g vs 167.38 μM TE/g of dry matter, respectively) (p<0.05). The peptide sequence of P3 was Cys-Cys-Cys-Cys-Ser-Val-Gln-Lys (888.30 Da). The novel peptide P3, at 250 μg/mL, also significantly inhibited HCT116 cell proliferation up to 25.24% through phosphorylation of ERK, JNK, and p38 kinase (p<0.05). Hence, antioxidant peptide P3 from Hanwoo beef extract can be used as an antioxidative and anti-cancer agent in the functional food industry.
Keyphrases
- anti inflammatory
- cell proliferation
- oxidative stress
- endothelial cells
- water soluble
- signaling pathway
- amino acid
- induced pluripotent stem cells
- high throughput
- risk assessment
- climate change
- small molecule
- induced apoptosis
- pluripotent stem cells
- heat shock protein
- tyrosine kinase
- human health
- endoplasmic reticulum stress