The Conserved Tetratricopeptide Repeat-Containing C-Terminal Domain of Pseudomonas aeruginosa FimV Is Required for Its Cyclic AMP-Dependent and -Independent Functions.
Ryan N C BuensucesoYlan NguyenKun ZhangMartin Daniel-IvadSeiji N Sugiman-MarangosAaron D FleetwoodIgor B ZhulinMurray S JunopP Lynne HowellLori L BurrowsPublished in: Journal of bacteriology (2016)
FimV is important for twitching motility and cAMP-dependent virulence gene expression in P. aeruginosa FimV homologs have been identified in several human pathogens, and their functions are not limited to T4P expression. The C terminus of FimV is remarkably conserved among otherwise very diverse family members, but its role is unknown. We provide here biological evidence for the importance of the C-terminal domain in both cAMP-dependent (through FimL) and -independent functions of FimV. We present X-ray crystal structures of the conserved C-terminal domain and identify a consensus sequence for the C-terminal TPR within the conserved domain. Our data extend our knowledge of FimV's functionally important domains, and the structures and consensus sequences provide a foundation for studies of FimV and its homologs.
Keyphrases
- pseudomonas aeruginosa
- transcription factor
- gene expression
- biofilm formation
- protein kinase
- binding protein
- high resolution
- endothelial cells
- cystic fibrosis
- healthcare
- escherichia coli
- poor prognosis
- antimicrobial resistance
- dna methylation
- staphylococcus aureus
- magnetic resonance
- machine learning
- electronic health record
- magnetic resonance imaging
- big data
- induced pluripotent stem cells
- acinetobacter baumannii
- long non coding rna
- pluripotent stem cells
- amino acid