Phylogenetic Analysis and Characterization of Diguanylate Cyclase and Phosphodiesterase in Planktonic Filamentous Cyanobacterium Arthrospira sp.
Kang WangWenjun LiHongli CuiSong QinPublished in: International journal of molecular sciences (2023)
Cyclic di-GMP (c-di-GMP) is a second messenger of intracellular communication in bacterial species, which widely modulates diverse cellular processes. However, little is known about the c-di-GMP network in filamentous multicellular cyanobacteria. In this study, we preliminarily investigated the c-di-GMP turnover proteins in Arthrospira based on published protein data. Bioinformatics results indicate the presence of at least 149 potential turnover proteins in five Arthrospira subspecies. Some proteins are highly conserved in all tested Arthrospira , whereas others are specifically found only in certain subspecies. To further validate the protein catalytic activity, we constructed a riboswitch-based c-di-GMP expression assay system in Escherichia coli and confirmed that a GGDEF domain protein, Adc11, exhibits potential diguanylate cyclase activity. Moreover, we also evaluated a protein with a conserved HD-GYP domain, Ahd1, the expression of which significantly improved the swimming ability of E. coli . Enzyme-linked immunosorbent assay also showed that overexpression of Ahd1 reduced the intracellular concentration of c-di-GMP, which is presumed to exhibit phosphodiesterase activity. Notably, meta-analyses of transcriptomes suggest that Adc11 and Ahd1 are invariable. Overall, this work confirms the possible existence of a functional c-di-GMP network in Arthrospira , which will provide support for the revelation of the biological function of the c-di-GMP system in Arthrospira .
Keyphrases
- biofilm formation
- escherichia coli
- pseudomonas aeruginosa
- staphylococcus aureus
- candida albicans
- binding protein
- poor prognosis
- protein protein
- high throughput
- cystic fibrosis
- meta analyses
- systematic review
- transcription factor
- randomized controlled trial
- amino acid
- electronic health record
- small molecule
- magnetic resonance
- risk assessment
- single cell
- computed tomography
- diffusion weighted
- climate change
- big data
- genetic diversity
- single molecule