NmeCas9 is an intrinsically high-fidelity genome-editing platform.
Nadia AmraniXin D GaoPengpeng LiuAlireza EdrakiAamir MirRaed IbraheimAnkit GuptaKanae E SasakiTong WuPaul D DonohoueAlexander H SettleAlexandra M LiedKyle McGovernChris K FullerPeter CameronThomas G FazzioLihua Julie ZhuScot A WolfeErik J SontheimerPublished in: Genome biology (2018)
Our results show that NmeCas9 is a naturally high-fidelity genome-editing enzyme and suggest that additional Cas9 orthologs may prove to exhibit similarly high accuracy, even without extensive engineering.
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