Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality.

Cláudia Lima Verde LealKarina Cañón-BeltránYulia N CajasMeriem HamdiAracelli YaryesMaría Gemma Millán de la BlancaPaula Beltrán-BreñaRosane MazzarellaJuliano Coelho da SilveiraAlfonso Gutiérrez-AdánEncina M GonzálezDimitrios Rizos

Published in: Journal of animal science and biotechnology (2022)

Mimicking physiological conditions using EVs from OF and UF in sequential IVC does not affect embryo development but improves blastocyst quality regarding survival rate after vitrification/warming, total cell number, lipid content, and relative changes in expression of lipid metabolism transcripts and lipase activation. Finally, EVs miRNA contents may contribute to the observed effects.

Keyphrases

poor prognosis
single cell
fatty acid
quality improvement
pregnancy outcomes
inferior vena cava
cell therapy
binding protein
pregnant women
mesenchymal stem cells
bone marrow
free survival