Metabolomic Profiles in the Brains of Juvenile Steelhead (Oncorhynchus mykiss) Following Bifenthrin Treatment.
Jason T MagnusonZachary CryderNicolette E AndrzejczykGary HarrakaDouglas C WolfJay GanDaniel SchlenkPublished in: Environmental science & technology (2020)
The pyrethroid insecticide, bifenthrin, is frequently measured at concentrations exceeding those that induce acute and chronic toxicity to several invertebrate and fish species residing in the Sacramento-San Joaquin Delta of California. Since the brain is considered to be a significant target for bifenthrin toxicity, juvenile steelhead trout (Oncorhynchus mykiss) were treated with concentrations of bifenthrin found prior to (60 ng/L) and following (120 ng/L) major stormwater runoff events with nontargeted metabolomics used to target transcriptomic alterations in steelhead brains following exposure. Predicted responses were involved in cellular apoptosis and necrosis in steelhead treated with 60 ng/L bifenthrin using the software Ingenuity Pathway Analysis. These responses were predominately driven by decreased levels of acetyl-l-carnitine (ALC), docosahexaenoic acid (DHA), and adenine. Steelhead treated with 120 ng/L bifenthrin had reductions of lysophosphatidylcholines (LPC), lysophosphatidylethanolamines (LPE), and increased levels of betaine, which were predicted to induce an inflammatory response. Several genes predicted to be involved in apoptotic (caspase3 and nrf2) and inflammatory (miox) pathways had altered expression following exposure to bifenthrin. There was a significantly increased expression of caspase3 and miox in fish treated with 120 ng/L bifenthrin with a significant reduction of nrf2 in fish treated with 60 ng/L bifenthrin. These data indicate that bifenthrin may have multiple targets within the brain that affect general neuron viability, function, and signaling potentially through alterations in signaling fatty acids.
Keyphrases
- oxidative stress
- cell death
- inflammatory response
- fatty acid
- poor prognosis
- newly diagnosed
- induced apoptosis
- mass spectrometry
- white matter
- endoplasmic reticulum stress
- liver failure
- electronic health record
- high resolution
- intensive care unit
- resting state
- zika virus
- genome wide
- big data
- cell proliferation
- toll like receptor
- acute respiratory distress syndrome
- cerebral ischemia
- signaling pathway
- artificial intelligence
- lps induced
- tandem mass spectrometry