Electroacupuncture promotes nerve regeneration and functional recovery in rats with spinal cord contusion through the coordinate interaction of CD4 and BDNF.
Bao-Lei ZhangXi-Liang GuoPublished in: Ibrain (2022)
To explore the effect of electroacupuncture on spinal cord injury (SCI) involving immune-related factors and regeneration-related factors in rats. The model of spinal cord contusion was established by PCI 3000 instrument. Two types of acupuncture points were selected for electroacupuncture treatment on rats. The rats were tested once a week, and the fiber remodeling was detected by magnetic resonance imaging. Transcriptome sequencing was performed on spinal scar samples. Using Python to write code, statistical analysis and bioinformatics analysis of the correlation between transcriptome sequencing data and fiber reconstruction results are carried out. Lastly, the expression of CD4 and brain-derived neurotrophic factor (BDNF) in spinal cord scar was verified by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Electroacupuncture exhibited a positive effect on the recovery of motor function in rats after SCI. Bioinformatics analysis found a direct interaction between CD4 and BDNF. Transcriptome sequencing and PCR results verified that electroacupuncture significantly reduced the expression of CD4, and increased significantly the expression of BDNF, simultaneously corresponding to nerve regeneration in rats with SCI. Our results showed that electroacupuncture intervention in SCI rats improves neural behavior via inhibiting the expression of CD4 and increasing the expression of BDNF.
Keyphrases
- spinal cord
- spinal cord injury
- neuropathic pain
- poor prognosis
- single cell
- magnetic resonance imaging
- stem cells
- rna seq
- randomized controlled trial
- stress induced
- computed tomography
- genome wide
- cerebral ischemia
- transcription factor
- coronary artery disease
- magnetic resonance
- percutaneous coronary intervention
- long non coding rna
- acute coronary syndrome
- mass spectrometry
- clinical trial
- artificial intelligence
- high resolution
- signaling pathway
- brain injury
- coronary artery bypass grafting
- blood brain barrier
- patient reported outcomes
- st segment elevation myocardial infarction