The indigoidine synthetase BpsA provides a colorimetric ATP assay that can be adapted to quantify the substrate preferences of other NRPS enzymes.
Alistair S BrownMark J CalcottVincent M CollinsJeremy G OwenDavid F AckerleyPublished in: Biotechnology letters (2020)
The robust nature and simplicity of the reaction protocol offers advantages over existing methods for ATP quantification and NRPS substrate analysis.