The mixture of cashew nut shell liquid and castor oil results in an efficient larvicide against Aedes aegypti that does not alter embryo-fetal development, reproductive performance or DNA integrity.
Juliana Miron VaniMaria Tereza Ferreira Duenhas MonrealSarah Alves AuharekAndréa Luiza Cunha-LauraEduardo José de ArrudaAlessandra Ramos LimaCicera Maria da SilvaAndréia Conceição Milan Brochado Antoniolli-SilvaDênis Pires de LimaAdilson BeatrizRodrigo Juliano OliveiraPublished in: PloS one (2018)
Dengue fever, chikungunya fever and Zika virus are epidemics in Brazil that are transmitted by mosquitoes, such as Aedes aegypti or Aedes albopictus. The liquid from shells of cashew nuts is attractive for its important biological and therapeutic activities, which include toxicity to mosquitoes of the genus Aedes. The present study evaluated the effects of a mixture of surfactants from natural cashew nutshell liquid and castor oil (named TaLCC-20) on the mortality of larvae and on the reproductive performance, embryonic and fetal development and genetic stability of Swiss mice. A total of 400 Ae. aegypti larvae (third larval stage) were treated with TaLCC-20 concentrations of 0.05 mg/L, 0.5 mg/L, or 5 mg/L (ppm). Twenty pregnant female mice were also orally administered TaLCC-20 at doses of 5 mg/kg and 50 mg/kg body weight (b.w.), and 10 animals were given only drinking water at 0.1 mL/10 g b.w. (orally). The results of a larvicide test demonstrated that 5 mg/mL TaLCC-20 killed 100% of larvae within three hours, which is comparable to the gold standard indicated by the Ministry of Health. Overall, these results show that TaLCC-20 is an efficient larvicide that does not induce genetic damage. In addition, changes in reproductive performance and embryo-fetal development appear positive, and the formulation is cost effective. Therefore, TaLCC-20 is an important product in the exploration of natural larvicides and can assist in fighting mosquitos as vectors for dengue fever, chikungunya fever and Zika virus, which are emerging/re-emerging and require proper management to ensure minimal harm to the human population. Therefore, TaLCC-20 can be considered a key alternative to commercial products, which are effective yet toxigenic.
Keyphrases
- aedes aegypti
- zika virus
- dengue virus
- drinking water
- body weight
- ionic liquid
- public health
- oxidative stress
- genome wide
- endothelial cells
- pregnant women
- health risk
- dna methylation
- health risk assessment
- fatty acid
- single molecule
- metabolic syndrome
- copy number
- cardiovascular events
- cell free
- heavy metals
- pluripotent stem cells
- social media
- risk assessment
- gene expression
- adipose tissue
- climate change