Safety evaluation and anti-inflammatory activity of Lactobacillus johnsonii IDCC 9203 isolated from feces of breast-fed infants.
Olajide Emmanuel AdedejiSeung A ChaeO-Hyun BanWon Yeong BangHayoung KimHyeon Ji JeonChiemela Enyinnaya ChinmaJungwoo YangYoung Hoon JungPublished in: Archives of microbiology (2022)
This study evaluated the safety of Lactobacillus johnsonii IDCC 9203 and investigated its anti-inflammatory activity in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Genomic analysis revealed that this strain has no virulence and antibiotic resistance gene except tetW, which is a tetracycline resistance gene. Minimum inhibitory concentration data showed that the strain is resistant to tetracycline and aminoglycosides. Further analysis indicated that the transferability of the tetW gene is extremely low, and resistance to aminoglycosides is due to the intrinsic resistance of L. johnsonii IDCC 9203. Phenotypic safety assessment showed that the strain has neither β-hemolytic nor β-glucuronidase activity, and no biogenic amine production. When LPS-induced RAW 264.7 cells were treated with L. johnsonii IDCC 9203, the level of nitric oxide and expression of pro-inflammatory cytokines significantly decreased (p < 0.05). Therefore, L. johnsonii IDCC 9203 strain is considered as safe and beneficial probiotic for human consumption.
Keyphrases
- lps induced
- inflammatory response
- nitric oxide
- copy number
- genome wide
- genome wide identification
- endothelial cells
- induced apoptosis
- escherichia coli
- poor prognosis
- toll like receptor
- lactic acid
- staphylococcus aureus
- single cell
- cell cycle arrest
- biofilm formation
- cell death
- transcription factor
- machine learning
- cystic fibrosis
- anti inflammatory
- antimicrobial resistance
- long non coding rna
- oxidative stress
- atomic force microscopy