Chopped fibers and nano-hydroxyapatite enhanced silk fibroin porous hybrid scaffolds for bone augmentation.
Shue JinXiaoxue FuWei-Nan ZengAnjing ChenZhenyu LuoYubao LiZongke ZhouJidong LiPublished in: Journal of materials chemistry. B (2023)
Chopped fiber (CF)- and nano-hydroxyapatite (n-HA)-enhanced silk fibroin (SF) porous hybrid scaffolds (SHCF) were prepared by freeze-drying for bone augmentation. Compared with pristine SF scaffolds, the incorporation of CF and n-HA can significantly enhance the mechanical properties of the composite scaffold. The results of cell experiments and mouse subcutaneous implantation indicated that the SHCF could alleviate foreign body reactions (FBR) led by macrophages and neutrophils, promote the polarization of RAW264.7 cells to anti-inflammatory M2 macrophages, and inhibit the secretion of pro-inflammatory cytokine TNF-α. A rat femoral defect repair model and bulk-RNA-seq analysis indicated that the CF- and n-HA-enhanced SHCF promoted the proliferation and osteogenic differentiation of bone mesenchymal stem cells (BMSCs) by the upregulation of Capns1 expression and regulated the calcium signaling pathway to mediate osteogenesis-related cell behavior, subsequently promoting bone regeneration.
Keyphrases
- tissue engineering
- bone regeneration
- single cell
- rna seq
- signaling pathway
- cystic fibrosis
- induced apoptosis
- mesenchymal stem cells
- soft tissue
- poor prognosis
- bone mineral density
- cell therapy
- anti inflammatory
- rheumatoid arthritis
- oxidative stress
- pi k akt
- epithelial mesenchymal transition
- bone loss
- endoplasmic reticulum stress
- cell proliferation
- cell death
- highly efficient
- drug induced
- lactic acid