Seminal Plasma Transcriptome and Proteome: Towards a Molecular Approach in the Diagnosis of Idiopathic Male Infertility.
Rossella CannarellaFederica BarbagalloAndrea CrafaSandro La VigneraRosita Angela CondorelliAldo Eugenio CalogeroPublished in: International journal of molecular sciences (2020)
As the "-omic" technology has largely developed, its application in the field of medical science seems a highly promising tool to clarify the etiology, at least in part, of the so-called idiopathic male infertility. The seminal plasma (SP) is made-up of secretions coming from the male accessory glands, namely epididymis, seminal vesicles, and prostate. It is not only a medium for sperm transport since it is able to modulate the female reproductive environment and immunity, to allow the acquisition of sperm competence, to influence the sperm RNA content, and even embryo development. The aim of this systematic review was to provide an updated and comprehensive description of the main transcripts and proteins reported by transcriptome and proteome studies performed in the human SP of patients with idiopathic infertility, in the attempt of identifying possible candidate molecular targets. We recurrently found that micro RNA (miR)-34, miR-122, and miR-509 are down-regulated in patients with non-obstructive azoospermia (NOA) and oligozoospermia compared with fertile controls. These molecules may represent interesting targets whose predictive role in testicular sperm extraction (TESE) or assisted reproductive techniques (ART) outcome deserves further investigation. Furthermore, according to the available proteomic studies, ECM1, TEX101, lectingalactoside-binding andsoluble 3 binding protein (LGALS3BP) have been reported as accurate predictors of TESE outcome. Interestingly, ECM1 is differently expressed in patients with different ART outcomes. Further prospective, ample-sized studies are needed to validate these molecular targets that will help in the counseling of patients with NOA or undergoing ART.
Keyphrases
- cell proliferation
- long non coding rna
- systematic review
- binding protein
- long noncoding rna
- case control
- hiv infected
- prostate cancer
- gene expression
- polycystic ovary syndrome
- rna seq
- single cell
- genome wide
- antiretroviral therapy
- endothelial cells
- public health
- meta analyses
- single molecule
- healthcare
- extracellular matrix
- metabolic syndrome
- type diabetes
- pregnant women
- randomized controlled trial
- human immunodeficiency virus
- benign prostatic hyperplasia
- adipose tissue
- mass spectrometry
- pluripotent stem cells
- dna methylation
- skeletal muscle
- weight loss
- pregnancy outcomes