Agrobacterium-mediated in planta transformation of cut coleoptile: a new, simplified, and tissue culture-independent method to deliver the CRISPR/Cas9 system in rice.
Amin-Asyraf TamiziAnis Afuza Md-YusofNurul Asyikin Mohd-ZimNazrul Hisham NazaruddinRogayah SekeliZarina ZainuddinNurul Hidayah SamsulrizalPublished in: Molecular biology reports (2023)
This protocol provides a rapid method for editing the rice genome, bypassing the need for tissue culture. This article is the first to report the delivery of the CRISPR/Cas9 system for in planta transformation in rice.