Targeted amplification-based whole genome sequencing of Monkeypox virus in clinical specimens.

Sandra Isabel AliReza EshaghiV R DuvvuriJonathan B GubbayK CroninAimin LiM HassoS T ClarkJ P HopkinsS N PatelThomas W A Braukmann

Published in: Microbiology spectrum (2023)

We present a protocol to efficiently sequence genomes of the MPXV-causing mpox. This enables researchers and public health agencies to acquire high-quality genomic data using a rapid and cost-effective approach. Genomic data can be used to conduct surveillance and investigate mpox outbreaks. We present 91 mpox genomes that show the diversity of the 2022 mpox outbreak in Ontario, Canada.

Keyphrases

public health
electronic health record
big data
copy number
randomized controlled trial
global health
data analysis
machine learning
nucleic acid
drug delivery