OleD Loki as a Catalyst for Hydroxamate Glycosylation.
Ryan R HughesKhaled A ShaabanLarissa V PonomarevaJamie HornChunhui ZhangChang-Guo ZhanS Randal VossMarkos LeggasJon S ThorsonPublished in: Chembiochem : a European journal of chemical biology (2019)
Herein we describe the ability of the permissive glycosyltransferase (GT) OleD Loki to convert a diverse set of >15 histone deacetylase (HDAC) inhibitors (HDACis) into their corresponding hydroxamate glycosyl esters. Representative glycosyl esters were subsequently evaluated in assays for cancer cell line cytotoxicity, chemical and enzymatic stability, and axolotl embryo tail regeneration. Computational substrate docking models were predictive of enzyme-catalyzed turnover and suggest certain HDACis may form unproductive, potentially inhibitory, complexes with GTs.
Keyphrases
- histone deacetylase
- room temperature
- papillary thyroid
- stem cells
- molecular dynamics
- squamous cell
- molecular dynamics simulations
- ionic liquid
- high throughput
- bone mineral density
- hydrogen peroxide
- cross sectional
- lymph node metastasis
- reduced graphene oxide
- carbon dioxide
- squamous cell carcinoma
- childhood cancer
- amino acid
- young adults
- gold nanoparticles
- single cell
- wound healing