μF-hBBB Chip Together with Tetrahedral DNA Frameworks for Visualization of LPS-Mediated Inflammation.
Yi XuShuainan LiChenguang WangXiaoming XieXianqiang MiPublished in: Analytical chemistry (2023)
The blood-brain barrier (BBB) is essential for maintaining central nervous system (CNS) stability, and neuroinflammation may cause the dysfunction of the BBB. MicroRNA-146a (miR-146a) is closely associated with neuroinflammation, which showed significant upregulation in response to lipopolysaccharide (LPS) induction. Elucidating the relationship between LPS-induced miR-146a expression and the BBB could decipher the mechanism of many neurological diseases. Here, we constructed an in vitro microfluidic human-BBB (μF-hBBB) chip consisting of human umbilical vein vascular endothelial cells (HUVECs) and human astrocyte (HAs) cells. A tetrahedral DNA framework (TDF-3MB) nanoprobe was used to label miR-146a in HUVECs on μF-hBBB chips before and after LPS induction, and the study revealed a significant increase in miR-146a expression after LPS induction. We believe that such a μF-hBBB chip is a promising in vitro platform for further use in understanding CNS diseases.
Keyphrases
- lps induced
- inflammatory response
- blood brain barrier
- endothelial cells
- cell proliferation
- long non coding rna
- poor prognosis
- lipopolysaccharide induced
- high throughput
- long noncoding rna
- circulating tumor cells
- toll like receptor
- anti inflammatory
- cerebral ischemia
- circulating tumor
- oxidative stress
- single cell
- induced apoptosis
- traumatic brain injury
- single molecule
- binding protein
- induced pluripotent stem cells
- cell free
- signaling pathway
- wastewater treatment
- immune response
- pluripotent stem cells
- living cells
- pi k akt