RNA-guided gene activation by CRISPR-Cas9-based transcription factors.
Pablo Perez-PineraD Dewran KocakChristopher M VockleyAndrew F AdlerAmi M KabadiLauren R PolsteinPratiksha I ThakoreKatherine A GlassDavid G OusteroutKam W LeongFarshid GuilakGregory E CrawfordTimothy E ReddyCharles A GersbachPublished in: Nature methods (2013)
Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation.
Keyphrases
- dna binding
- transcription factor
- genome wide identification
- crispr cas
- genome editing
- genome wide
- nucleic acid
- copy number
- poor prognosis
- healthcare
- magnetic resonance
- high glucose
- diabetic rats
- dna methylation
- binding protein
- magnetic resonance imaging
- genome wide analysis
- long non coding rna
- single molecule
- cell free
- gene expression
- computed tomography
- network analysis