Programmable multi-kilobase RNA editing using CRISPR-mediated trans-splicing.

Jacob Borrajo Kamyab Javanmardi James Griffin Susan J St Martin David Yao Kaisle A Hill Paul C Blainey Basem Al-Shayeb

Published in: bioRxiv : the preprint server for biology (2023)

CRISPR-guided trans-splicing enables efficient and specific recombination of large RNA molecules in mammalian cells, with broad applications in therapeutic development for genetic diseases and as a research tool for the study of basic RNA biology.

Keyphrases

crispr cas
genome wide
genome editing
dna damage
dna methylation
dna repair
gene expression