A novel approach to single-cell analysis reveals intrinsic differences in immune marker expression in unstimulated BALB/c and C57BL/6 macrophages.
Jeremie BredaArka BanerjeeRajesh JayachandranJean PietersMihaela ZavolanPublished in: FEBS letters (2022)
The origin of functional heterogeneity among macrophages, key innate immune system components, is still debated. While mouse strains differ in their immune responses, the range of gene expression variation among their pre-stimulation macrophages is unknown. With a novel approach to scRNA-seq analysis, we reveal the gene expression variation in unstimulated macrophage populations from BALB/c and C57BL/6 mice. We show that intrinsic strain-to-strain differences are detectable before stimulation and we place the unstimulated single cells within the gene expression landscape of stimulated macrophages. C57BL/6 mice show stronger evidence of macrophage polarization than BALB/c mice, which may contribute to their relative resistance to pathogens. Our computational methods can be generally adopted to uncover biological variation between cell populations.
Keyphrases
- single cell
- gene expression
- rna seq
- immune response
- dna methylation
- high fat diet induced
- high throughput
- genome wide
- induced apoptosis
- poor prognosis
- escherichia coli
- type diabetes
- skeletal muscle
- wild type
- toll like receptor
- insulin resistance
- long non coding rna
- multidrug resistant
- gram negative
- antimicrobial resistance
- signaling pathway
- mesenchymal stem cells
- cell proliferation
- cell therapy
- dendritic cells
- cell cycle arrest
- endoplasmic reticulum stress
- bone marrow