In Vivo Luciferin-Luciferase Reaction in Micro-Mini Pigs Using Xenogeneic Rat Bone Marrow Transplantation.
Tomoyuki AbeKazuhiro EndoYutaka HanazonoKobayashi EijiPublished in: International journal of molecular sciences (2024)
Luminescent technology based on the luciferin-luciferase reaction has been extensively employed across various disciplines as a quantitative imaging modality. Owing to its non-invasive imaging capacity, it has evolved as a valuable in vivo bioimaging tool, particularly in small animal models in fields such as gene and cell therapies. We have previously successfully generated rats with a systemic expression of the luciferase gene at the Rosa26 locus. In this study, we transplanted bone marrow from these rats into micro-mini pigs and used in vivo imaging to non-invasively analyze the dynamics of the transplanted cells. In addition, we established that the rat-to-pig transplantation system is a discordant system, similar to the pig-to-human transplantation system. Thus, rat-to-pig transplantation may provide a clinically appropriate large animal model for pig-to-human xenotransplantation.
Keyphrases
- bone marrow
- high resolution
- cell therapy
- endothelial cells
- oxidative stress
- mesenchymal stem cells
- induced pluripotent stem cells
- induced apoptosis
- copy number
- quantum dots
- poor prognosis
- pluripotent stem cells
- gene expression
- multidrug resistant
- fluorescence imaging
- signaling pathway
- dna methylation
- fluorescent probe
- photodynamic therapy
- drug induced