Modification of the effects of aflatoxin B1 on the glutathione system and its regulatory genes by zeolite.
Benjámin KövesiSzabina KulcsárMátyás CserhátiMárta ErdélyiZsolt AncsinErika ZándokiMiklós MézesKrisztián BaloghPublished in: Acta veterinaria Hungarica (2021)
The purpose of the present study was to use oxidative stress markers for investigating the effect of zeolite (315 mg/kg of complete feed) in the case of aflatoxin B1 contamination (92 μg/kg complete feed). In a 21-day feeding trial with broiler chickens, oxidative stress parameters such as conjugated dienes, conjugated trienes, malondialdehyde, reduced glutathione content and glutathione peroxidase activity were not changed significantly by supplementation with this mycotoxin absorbent. The relative gene expression of transcription factors KEAP1 and NRF2 was not modified by the absorbent either. Still, the expression of GSS, GSR and GPX4 genes increased significantly due to the aluminosilicate supplementation. The results suggest that zeolite reduced lipid peroxidation in the blood plasma but not in the red blood cell haemolysate or the kidney. The relative expression of the genes encoding the glutathione redox system also changed as a result of zeolite supplementation, but these changes were not found at the protein level.
Keyphrases
- oxidative stress
- red blood cell
- gene expression
- genome wide identification
- transcription factor
- poor prognosis
- genome wide
- bioinformatics analysis
- binding protein
- photodynamic therapy
- dna damage
- clinical trial
- risk assessment
- diabetic rats
- induced apoptosis
- study protocol
- protein protein
- drinking water
- randomized controlled trial
- hydrogen peroxide
- nitric oxide
- phase ii
- phase iii
- heat shock
- heavy metals