A facile chemoenzymatic synthesis of SARS-CoV-2 glycopeptides for probing glycosylation functions.
Guanghui ZongChao LiSunaina Kiran PrabhuRoushu ZhangXiao ZhangLai-Xi WangPublished in: Chemical communications (Cambridge, England) (2021)
Glycosylation plays important roles in SARS-CoV-2 infection. We describe here a facile chemoenzymatic synthesis of core-fucosylated N-glycopeptides derived from the SARS-CoV-2 Spike protein and their binding with glycan-dependent neutralizing antibody S309 and human lectin CLEC4G. The synthetic glycopeptides provide tools for further functional characterization of viral glycosylation.
Keyphrases
- sars cov
- respiratory syndrome coronavirus
- endothelial cells
- quantum dots
- reduced graphene oxide
- highly efficient
- binding protein
- induced pluripotent stem cells
- dengue virus
- coronavirus disease
- pluripotent stem cells
- dna binding
- visible light
- zika virus
- gold nanoparticles
- small molecule
- cell surface
- transcription factor