CRISPR screens are feasible in TP53 wild-type cells.

Kevin R Brown Barbara Mair Martin Soste Jason Moffat

Published in: Molecular systems biology (2020)

A recent study by Haapaniemi et al (2018) reported that intact p53 signaling hampers CRISPR-based functional genomic screens. Brown et al report good performance of genome-scale screens in TP53 wild-type cells and reiterate best practices for CRISPR screening.

Keyphrases

genome wide
wild type
induced apoptosis
dna methylation
crispr cas
genome editing
cell cycle arrest
copy number
high throughput
healthcare
endoplasmic reticulum stress
primary care
gene expression
cell death
pi k akt