Functional assessment of plant and microalgal lipid pathway genes in yeast to enhance microbial industrial oil production.
Huadong PengLalehvash MoghaddamAnthony BrininBrett WilliamsSagadevan MundreeVictoria S HaritosPublished in: Biotechnology and applied biochemistry (2017)
As promising alternatives to fossil-derived oils, microbial lipids are important as industrial feedstocks for biofuels and oleochemicals. Our broad aim is to increase lipid content in oleaginous yeast through expression of lipid accumulation genes and use Saccharomyces cerevisiae to functionally assess genes obtained from oil-producing plants and microalgae. Lipid accumulation genes DGAT (diacylglycerol acyltransferase), PDAT (phospholipid: diacylglycerol acyltransferase), and ROD1 (phosphatidylcholine: diacylglycerol choline-phosphotransferase) were separately expressed in yeast and lipid production measured by fluorescence, solvent extraction, thin layer chromatography, and gas chromatography (GC) of fatty acid methyl esters. Expression of DGAT1 from Arabidopsis thaliana effectively increased total fatty acids by 1.81-fold above control, and ROD1 led to increased unsaturated fatty acid content of yeast lipid. The functional assessment approach enabled the fast selection of candidate genes for metabolic engineering of yeast for production of lipid feedstocks.
Keyphrases
- fatty acid
- saccharomyces cerevisiae
- gas chromatography
- genome wide
- mass spectrometry
- poor prognosis
- bioinformatics analysis
- tandem mass spectrometry
- genome wide identification
- cell wall
- wastewater treatment
- microbial community
- heavy metals
- genome wide analysis
- gene expression
- liquid chromatography
- binding protein
- dna methylation
- gas chromatography mass spectrometry
- high resolution