Effects and Stress-Relieving Mechanisms of Dark Tea Polysaccharide in Human HaCaT Keratinocytes and SZ95 Sebocytes.
Chang GaoJiafeng FuJunyi CuiTingzhi ZhangChristos C ZouboulisJing WangShaowei YanPublished in: Molecules (Basel, Switzerland) (2023)
A new skincare application scenario for dark tea, a unique and post-fermented tea popular in the health food industry, was developed in this paper. The effects of dark tea polysaccharide (DTP) on stress-induced skin problems and its mechanism of action were investigated by modeling cortisone-induced stress injury in human HaCaT keratinocytes and SZ95 sebaceous gland cells. The results showed a reduced cortisol conversion induced by cortisone under the action of DTP with a concentration of 200 μg/mL, probably by inhibiting the expression of the HSD11B1 enzyme. DTP was also able to suppress the cortisone-induced elevation of lipid levels in SZ95 sebocytes at this concentration. In addition, the composition and structure of DTP were verified by ultrafiltration, ultraviolet-visible spectrophotometry (UV-VIS), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and infrared spectroscopy. In brief, DTP has a unique and significant stress-relieving effect, which provides new ideas for the development of new ingredients for the skin care industry.
Keyphrases
- stress induced
- endothelial cells
- high glucose
- healthcare
- wound healing
- mental health
- diabetic rats
- induced pluripotent stem cells
- poor prognosis
- induced apoptosis
- public health
- soft tissue
- drug induced
- pluripotent stem cells
- palliative care
- signaling pathway
- oxidative stress
- human health
- health information
- nitric oxide
- risk assessment
- chronic pain
- real time pcr
- heat stress
- cell death
- ms ms
- endoplasmic reticulum stress
- reduced graphene oxide
- aqueous solution