Mesenchymal Stem Cells Decrease Oxidative Stress in the Bowels of Interleukin-10 Knockout Mice.
Kyong Jin JungGun-Woo LeeChul-Hyun ParkTae-Jin LeeJoo Young KimEon Gi SungSeong Yong KimByeong Ik JangIn Hwan SongPublished in: Gut and liver (2020)
<b>Background/Aims:</b> Inflammatory bowel disease (IBD) is an autoimmune disease characterized by chronic inflammation mainly in the large intestine. The interleukin-10 knockout (IL-10 KO) mouse is a well-known animal model of IBD that develops spontaneous intestinal inflammation resembling Crohn's disease. Oxidative stress is considered to be the leading cause of cell and tissue damage. Reactive oxygen species (ROS) can cause direct cell injury and/or indirect cell injury by inducing the secretion of cytokines from damaged cells. This study evaluated the effects of mesenchymal stem cell (MSC) on the progression of IBD. <b>Methods:</b> In this study, human bone marrow-derived MSCs were injected into IL-10 KO mice (MSC). Oxidative stress and inflammation levels were evaluated in the large intestine and compared with those in control IL-10 KO mice (CON) and normal wild-type control mice (Wild). <b>Results:</b> The levels of ROS (superoxide and hydrogen peroxidase) and a secondary end-product of lipid peroxidation (malondialdehyde) were considerably higher in the CON, while superoxide dismutase and catalase levels were lower in the MSC. Inflammation-related marker (interferon-γ, tumor necrosis factor-α, IL-4, and CD8) expression and inflammatory histological changes were much less pronounced in MSC than in CON. <b>Conclusions:</b> MSCs affect the redox balance, leading to the suppression of IBD.
Keyphrases
- oxidative stress
- mesenchymal stem cells
- induced apoptosis
- wild type
- dna damage
- cell therapy
- reactive oxygen species
- umbilical cord
- diabetic rats
- single cell
- ischemia reperfusion injury
- bone marrow
- hydrogen peroxide
- high fat diet induced
- ulcerative colitis
- cell death
- endothelial cells
- poor prognosis
- multiple sclerosis
- stem cells
- type diabetes
- nitric oxide
- dendritic cells
- long non coding rna
- insulin resistance
- fatty acid
- skeletal muscle
- adipose tissue
- immune response
- binding protein