Toward Kilogram-Scale Peroxygenase-Catalyzed Oxyfunctionalization of Cyclohexane.

Mol-scale oxyfunctionalization of cyclohexane to cyclohexanol/cyclohexanone (KA-oil) using an unspecific peroxygenase is reported. Using Aae UPO from Agrocybe aegerita and simple H 2 O 2 as an oxidant, cyclohexanol concentrations of more than 300 mM (>60% yield) at attractive productivities (157 mM h -1 , approx. 15 g L -1 h -1 ) were achieved. Current limitations of the proposed biooxidation system have been identified paving the way for future improvements and implementation.