Toxicity of Fe2 O3 nanoparticles on human blood lymphocytes.
Evelyn AssadianHamid DezhampanahEnayatollah SeydiJalal PourahmadPublished in: Journal of biochemical and molecular toxicology (2019)
Magnetic nanoparticles (NPs) are used to a large extent in the targeted delivery of therapeutic agents. In this study, we aimed to investigate the possible toxicity of Fe2 O 3 NPs on human cells, including blood lymphocytes. We isolated blood lymphocytes from healthy humans using Ficoll polysaccharide and subsequently by gradient centrifugation. Then, the toxicity parameters, including cell viability, reactive oxygen species (ROS) formation, lipid peroxidation, cellular glutathione (GSH) level, mitochondrial and lysosomal damage, were measured in blood lymphocytes after exposure to Fe 2 O 3 NPs. Our results indicated that Fe 2 O 3 NPs significantly (dependent on concentration) reduced the cell viability, and the IC 50 was determined to be 1 mM. With increasing concentrations, we found that Fe 2 O 3 NPs-induced cell toxicity was associated with a significant increase in intracellular ROS and loss of mitochondrial membrane potential and lysosomal membrane leakiness. Consequently, these NPs at different concentrations affect GSH level and cause oxidative stress in human lymphocytes.
Keyphrases
- oxidative stress
- oxide nanoparticles
- reactive oxygen species
- peripheral blood
- diabetic rats
- dna damage
- endothelial cells
- magnetic nanoparticles
- induced apoptosis
- induced pluripotent stem cells
- pluripotent stem cells
- risk assessment
- single cell
- cell therapy
- fatty acid
- heat shock
- drug induced
- signaling pathway
- climate change
- endoplasmic reticulum stress
- water soluble