Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression.
Fatemeh AzadmaneshMehrab PourmadadiJavad Zavar RezaFatemeh YazdianMeisam OmidiBibi Fatemeh HaghirosadatPublished in: Biotechnology progress (2021)
In this study, a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold fabricated using poly-lactic acid (PLA). The core of scaffold was composed of hyaluronic acid (HA), copper carbon dots (Cu-CDs), rosmarinic acid, and chitosan hydrogel. Cu-CDs were synthesized using ammonium hydrogen citrate under hydrothermal conditions. Formulation containing 1 mg ml-1 concentration of Cu-CDs showed an excellent antibacterial activity against gram bacteria. At 0.25 mg ml-1 of Cu-CDs concentration, scaffold had a good biocompatibility as confirmed by cytotoxicity assay on L929 fibroblast stem cells. in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold. This finding was confirmed by histological analysis and the relative expression of PDGF, TGF-β, and MMP-1 genes. The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.
Keyphrases
- wound healing
- quantum dots
- tissue engineering
- visible light
- stem cells
- gene expression
- hyaluronic acid
- aqueous solution
- metal organic framework
- drug delivery
- lactic acid
- poor prognosis
- single cell
- genome wide
- signaling pathway
- gram negative
- replacement therapy
- multidrug resistant
- gold nanoparticles
- binding protein
- silver nanoparticles
- transcription factor
- anaerobic digestion