Transcriptional profile of the rat cardiovascular system at single cell resolution.
Alessandro ArduiniStephen J FlemingLing XiaoAmelia Weber HallAmer-Denis AkkadMark D ChaffinKayla J BendinelliNathan R TuckerIrinna PapangeliHelene MantineoMehrtash BabadiChristian M StegmannGuillermo García-CardeñaMark E LindsayCarla KlattenhoffPatrick T EllinorPublished in: bioRxiv : the preprint server for biology (2023)
Through a large single nucleus sequencing effort encompassing over 500,000 nuclei, we broadened our understanding of cellular transcription in the healthy cardiovascular system. The existence of tissue-restricted cellular phenotypes suggests regional regulation of cardiovascular physiology. The overall conservation in gene expression and molecular pathways across rat and human cell types, together with our detailed transcriptional characterization of each cell type, offers the potential to identify novel therapeutic targets and improve preclinical models of cardiovascular disease.
Keyphrases
- single cell
- gene expression
- cardiovascular disease
- rna seq
- transcription factor
- endothelial cells
- oxidative stress
- cell therapy
- high throughput
- dna methylation
- type diabetes
- single molecule
- stem cells
- risk assessment
- heat shock
- cardiovascular risk factors
- cardiovascular events
- pluripotent stem cells
- induced pluripotent stem cells