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Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy.

Giuliano ScarcelliWilliam J PolacheckHadi T NiaKripa PatelAlan J GrodzinskyRoger D KammSeok Hyun Yun
Published in: Nature methods (2015)
Current measurements of the biomechanical properties of cells require physical contact with cells or lack subcellular resolution. Here we developed a label-free microscopy technique based on Brillouin light scattering that is capable of measuring an intracellular longitudinal modulus with optical resolution. The 3D Brillouin maps we obtained of cells in 2D and 3D microenvironments revealed mechanical changes due to cytoskeletal modulation and cell-volume regulation.
Keyphrases
  • induced apoptosis
  • label free
  • high resolution
  • cell cycle arrest
  • single cell
  • high speed
  • physical activity
  • high throughput
  • oxidative stress
  • cross sectional
  • reactive oxygen species
  • bone marrow