The molecular landscape of glioma in patients with Neurofibromatosis 1.
Fulvio D'AngeloMichele Ceccarellinull TalaLuciano GarofanoJing ZhangVéronique FrattiniFrancesca P CarusoGenevieve LewisKristin D AlfaroLuc BauchetGiulia BerzeroDavid CachiaMario CangianoLaurent CapelleJohn de GrootFrancesco DiMecoFrançois DucrayWalid FarahGaetano FinocchiaroStéphane GoutagnyCarlos Kamiya-MatsuokaCinzia LavarinoHugues LoiseauVéronique LorgisCarlo E MarrasIan E McCutcheonDo-Hyun NamSusanna RonchiVeronica SalettiRomuald SeizeurJohn SlopisMariona SuñolFanny VandenbosPascale VarletDominique VidaudColin WattsViviane TabarDavid E ReussSeung-Ki KimDavid MeyronetKarima MokhtariHector SalvadorKrishna P BhatMarica EoliMarc SansonAnna LasorellaAntonio IavaronePublished in: Nature medicine (2018)
Neurofibromatosis type 1 (NF1) is a common tumor predisposition syndrome in which glioma is one of the prevalent tumors. Gliomagenesis in NF1 results in a heterogeneous spectrum of low- to high-grade neoplasms occurring during the entire lifespan of patients. The pattern of genetic and epigenetic alterations of glioma that develops in NF1 patients and the similarities with sporadic glioma remain unknown. Here, we present the molecular landscape of low- and high-grade gliomas in patients affected by NF1 (NF1-glioma). We found that the predisposing germline mutation of the NF1 gene was frequently converted to homozygosity and the somatic mutational load of NF1-glioma was influenced by age and grade. High-grade tumors harbored genetic alterations of TP53 and CDKN2A, frequent mutations of ATRX associated with Alternative Lengthening of Telomere, and were enriched in genetic alterations of transcription/chromatin regulation and PI3 kinase pathways. Low-grade tumors exhibited fewer mutations that were over-represented in genes of the MAP kinase pathway. Approximately 50% of low-grade NF1-gliomas displayed an immune signature, T lymphocyte infiltrates, and increased neo-antigen load. DNA methylation assigned NF1-glioma to LGm6, a poorly defined Isocitrate Dehydrogenase 1 wild-type subgroup enriched with ATRX mutations. Thus, the profiling of NF1-glioma defined a distinct landscape that recapitulates a subset of sporadic tumors.
Keyphrases
- high grade
- low grade
- signaling pathway
- lps induced
- pi k akt
- nuclear factor
- genome wide
- dna methylation
- oxidative stress
- end stage renal disease
- newly diagnosed
- gene expression
- ejection fraction
- chronic kidney disease
- single cell
- prognostic factors
- clinical trial
- inflammatory response
- wild type
- randomized controlled trial
- transcription factor
- toll like receptor
- immune response
- patient reported
- study protocol
- patient reported outcomes