Metabolic transformation of fat in obesity determines the inflammation resolving capacity of splenocardiac and cardiorenal networks in heart failure.
Ganesh V HaladeVasundhara KainXavier De La RosaMerry L LindseyPublished in: American journal of physiology. Heart and circulatory physiology (2022)
All fats are not created equal, and despite the extensive literature, the effect of fat intake is the most debated question in obesity, cardiovascular, and cardiorenal research. Cellular and molecular mechanisms underlying cardiac dysfunction and consequent heart failure in the setting of obesity are not well understood. Our understanding of how fats are metabolically transformed after nonreperfused myocardial infarction (MI), in particular, is incomplete. Here, using male C57BL/6J mice (2 mo old), we determined the role of omega-6 fatty acids, provided as safflower oil (SO) for 12 wk, followed by supplementation with docosahexaenoic acid (DHA; n-3 fatty acids) for 8 wk before MI. With SO feeding, inflammation resolution was impaired. Specialized proresolving mediators (SPMs) increased in DHA-fed mice to reverse the effects of SO, whereas prostaglandins and thromboxane B 2 were reduced in the spleen and amplified multiple resolving mechanisms in heart and kidney post-MI. DHA amplified the number of resolving macrophages and cardiac reparative pathways of the splenocardiac and cardiorenal networks in acute heart failure, with higher T reg cells in chronic heart failure and marked expression of Foxp3 + in the myocardium. Our findings indicate that surplus ingestion of SO intensified systemic, baseline, nonresolving inflammation, and DHA intake dominates splenocardiac resolving phase with the biosynthesis of SPMs and controlled cardiorenal inflammation in heart failure survivor mice. NEW & NOTEWORTHY Chronic and surplus dietary intake of safflower oil (SO) increased plasma creatinine dysregulated post-MI splenocardiac inflammation coincides with the dysfunctional cardiorenal network. In contrast, docosahexaenoic acid (DHA) increases post-MI survival in chronic heart failure. DHA transforms into specialized proresolving mediators (SPMs) and limited proinflammatory prostaglandins and thromboxanes following myocardial infarction (MI). DHA promotes Ly6C low resolving macrophages and T regulatory cells (Foxp3 + ) in a splenocardiac manner post-MI.
Keyphrases
- fatty acid
- heart failure
- high fat diet induced
- oxidative stress
- acute heart failure
- left ventricular
- induced apoptosis
- insulin resistance
- metabolic syndrome
- weight gain
- type diabetes
- weight loss
- regulatory t cells
- atrial fibrillation
- magnetic resonance imaging
- palliative care
- cell cycle arrest
- poor prognosis
- cardiac resynchronization therapy
- adipose tissue
- magnetic resonance
- transcription factor
- computed tomography
- skeletal muscle
- cell death