APOBEC3 degradation is the primary function of HIV-1 Vif for virus replication in the myeloid cell line THP-1.

Terumasa IkedaRyo ShimizuHesham NasserMichael A CarpenterAdam Z ChengWilliam L BrownDaniel Sauter Reuben Stewart Harris

Published in: bioRxiv : the preprint server for biology (2023)

genes in the THP-1 myeloid cell line using CRISPR and compared the infectivity of wildtype HIV-1 and Vif mutants with the selective A3 neutralization activities. Our results demonstrate that the infectivity of Vif-deficient HIV-1 and the other Vif mutants is fully restored by ablating the expression of cellular A3A to A3G proteins. These results indicate that A3 proteins are the only essential target of Vif that is required for HIV-1 replication in THP-1 cells.

Keyphrases

antiretroviral therapy
hiv positive
hiv infected
hiv testing
human immunodeficiency virus
hiv aids
hepatitis c virus
men who have sex with men
induced apoptosis
poor prognosis
south africa
acute myeloid leukemia
dendritic cells
crispr cas
cell death
long non coding rna
binding protein
cell cycle arrest