Long noncoding RNA NONHSAT177112.1 aggravates inflammation and apoptosis in LPS-treated human cardiomyocytes.
Yaru KongBo HanLi ZhangQingqing LiuGuanlin ZhengKeying GuoQing ChenZhongmei ChenPublished in: Epigenomics (2021)
Aim: To explore the roles of lncRNA NONHSAT177112.1 in the inflammatory injury of human cardiomyocytes (HCMs) induced by lipopolysaccharide (LPS). Materials & methods: The sublocalization of NONHSAT177112.1 was detected by FISH. HCMs were stimulated with LPS to induce inflammatory injury. NONHSAT177112.1 expression was detected by quantitative real-time PCR. Cell apoptosis and viability were detected by flow cytometry and CCK-8 assays. The expression of inflammatory cytokines and myocardial enzymes were detected by PCR and ELISA. Results: NONHSAT177112.1 is expressed in the nucleus and cytoplasm. NONHSAT177112.1 showed dynamic expression that first increased and then decreased during LPS stimulation. NONHSAT177112.1 knockdown reversed the promotion effect of LPS on inflammatory injury. Conversely, NONHSAT177112.1 overexpression exerted the opposite effects. Conclusion: NONHSAT177112.1 aggravates inflammatory injury in LPS-treated HCMs.
Keyphrases
- inflammatory response
- oxidative stress
- anti inflammatory
- long noncoding rna
- poor prognosis
- endothelial cells
- flow cytometry
- real time pcr
- toll like receptor
- cell proliferation
- lps induced
- high resolution
- binding protein
- long non coding rna
- high throughput
- high glucose
- heart failure
- newly diagnosed
- pluripotent stem cells