Measurement of Metabolome Samples Using Liquid Chromatography-Mass Spectrometry, Data Acquisition, and Processing.
Tomáš PluskalMitsuhiro YanagidaPublished in: Cold Spring Harbor protocols (2016)
We present a protocol for metabolomic sample measurement using hydrophilic interaction chromatography (HILIC) combined with high-resolution Orbitrap mass spectrometry (MS). We also introduce a raw data processing method using MZmine 2 software, and include a list of 111 metabolite peaks (with their m/z values and retention times) previously identified in metabolome samples using this method.
Keyphrases
- mass spectrometry
- liquid chromatography
- high resolution
- tandem mass spectrometry
- high resolution mass spectrometry
- ultra high performance liquid chromatography
- gas chromatography
- high performance liquid chromatography
- electronic health record
- simultaneous determination
- capillary electrophoresis
- solid phase extraction
- big data
- randomized controlled trial
- data analysis
- multiple sclerosis
- artificial intelligence