Successful fishing for nucleus pulposus progenitor cells of the intervertebral disc across species.

Daisuke Sakai Jordy Schol Frances C Bach Adel Tekari Nobuho Sagawa Yoshihiko Nakamura Samantha C W Chan Tomoko Nakai Laura B Creemers Daniela A Frauchiger Rahel D May Sybille Grad Masahiko Watanabe Marianna A Tryfonidou Benjamin Gantenbein

Published in: JOR spine (2018)

Current identification of Tie2+ cells could be confirmed in bovine, canine, mouse, and human specimens. The presented flow cytometry protocol can successfully sort these multipotent cells. The biological function of isolated cells based on Tie2+ expression needs to be confirmed by functional assays such as in vitro differentiation. in vitro culture conditions to maintain and their possible proliferation of the Tie2+ fraction is the subject of future research.

Keyphrases

induced apoptosis
cell cycle arrest
flow cytometry
endothelial cells
poor prognosis
signaling pathway
randomized controlled trial
endoplasmic reticulum stress
oxidative stress
cell death
cell proliferation
pi k akt
finite element