Platelet Lysate for Mesenchymal Stromal Cell Culture in the Canine and Equine Species: Analogous but Not the Same.
Alina HagenHeidrun HollandVivian-Pascal BrandtCarla U DollThomas C HäußlerMichaela MelzerJulia MoellerberndtHendrik LehmannJanina BurkPublished in: Animals : an open access journal from MDPI (2022)
Platelet lysate (PL) is an attractive platelet-based therapeutic tool and has shown promise as xeno-free replacement for fetal bovine serum (FBS) in human and equine mesenchymal stromal cell (MSC) culture. Here, we established a scalable buffy-coat-based protocol for canine PL (cPL) production (n = 12). The cPL was tested in canine adipose MSC (n = 5) culture compared to FBS. For further comparison, equine adipose MSC (n = 5) were cultured with analogous equine PL (ePL) or FBS. During canine blood processing, platelet and transforming growth factor-β1 concentrations increased ( p < 0.05 and p < 0.001), while white blood cell concentrations decreased ( p < 0.05). However, while equine MSC showed good results when cultured with 10% ePL, canine MSC cultured with 2.5% or 10% cPL changed their morphology and showed decreased metabolic activity ( p < 0.05). Apoptosis and necrosis in canine MSC were increased with 2.5% cPL ( p < 0.05). Surprisingly, passage 5 canine MSC showed less genetic aberrations after culture with 10% cPL than with FBS. Our data reveal that using analogous canine and equine biologicals does not entail the same results. The buffy-coat-based cPL was not adequate for canine MSC culture, but may still be useful for therapeutic applications.
Keyphrases
- endothelial cells
- bone marrow
- transforming growth factor
- stem cells
- single cell
- randomized controlled trial
- epithelial mesenchymal transition
- type diabetes
- gene expression
- oxidative stress
- cell death
- big data
- copy number
- metabolic syndrome
- cell therapy
- dna methylation
- cell proliferation
- mesenchymal stem cells
- artificial intelligence
- machine learning
- pluripotent stem cells