Differential Distribution of the wlaN and cgtB Genes, Associated with Guillain-Barré Syndrome, in Campylobacter jejuni Isolates from Humans, Broiler Chickens, and Wild Birds.
Pedro GuiradoSonia PaytubiElisenda MiróYaidelis Iglesias-TorrensCristina MadridMarta Cerdà-CuéllarCamille Stephan-Otto AttoliniCarlos BalsalobreCristina MadridPublished in: Microorganisms (2020)
Campylobacter jejuni causes campylobacteriosis, a bacterial gastroenteritis with high incidence worldwide. Moreover, C. jejuni infection can trigger the polyneuropathic disorder denominated Guillain-Barré syndrome (GBS). The C. jejuni strains that can elicit GBS carry either wlaN or cgtB, coding both genes for a β-1,3-galactosyltransferase enzyme that is required for the production of sialylated lipooligosaccharide (LOSSIAL). We described a differential prevalence of the genes wlaN and cgtB in C. jejuni isolates from three different ecological niches: humans, broiler chickens, and wild birds. The distribution of both genes, which is similar between broiler chicken and human isolates and distinct when compared to the wild bird isolates, suggests a host-dependent distribution. Moreover, the prevalence of the wlaN and cgtB genes seems to be restricted to some clonal complexes. Gene sequencing identified the presence of new variants of the G- homopolymeric tract within the wlaN gene. Furthermore, we detected two variants of a G rich region within the cgtB gene, suggesting that, similarly to wlaN, the G-tract in the cgtB gene mediates the phase variation control of cgtB expression. Caco-2 cell invasion assays indicate that there is no evident correlation between the production of LOSSIAL and the ability to invade eukaryotic cells.
Keyphrases
- genome wide identification
- genome wide
- copy number
- genome wide analysis
- transcription factor
- dna methylation
- risk factors
- genetic diversity
- bioinformatics analysis
- case report
- escherichia coli
- poor prognosis
- single cell
- pseudomonas aeruginosa
- cell cycle arrest
- cell death
- pluripotent stem cells
- induced pluripotent stem cells