In Vitro and In Vivo Effects of Ulvan Polysaccharides from Ulva rigida .
Jorge García-MárquezBruna Rodrigues MoreiraPiedad Valverde-GuillénSofía Latorre-RedoliCandela T Caneda-SantiagoFrancisco Gabriel Acién-FernándézEduardo Martínez-ManzanaresManuel Marí-BeffaRoberto T Abdala-DíazPublished in: Pharmaceuticals (Basel, Switzerland) (2023)
One of the main bioactive compounds of interest from the Ulva species is the sulfated polysaccharide ulvan, which has recently attracted attention for its anticancer properties. This study investigated the cytotoxic activity of ulvan polysaccharides obtained from Ulva rigida in the following scenarios: (i) in vitro against healthy and carcinogenic cell lines (1064sk (human fibroblasts), HACAT (immortalized human keratinocytes), U-937 (a human leukemia cell line), G-361 (a human malignant melanoma), and HCT-116 (a colon cancer cell line)) and (ii) in vivo against zebrafish embryos. Ulvan exhibited cytotoxic effects on the three human cancer cell lines tested. However, only HCT-116 demonstrated sufficient sensitivity to this ulvan to make it relevant as a potential anticancer treatment, presenting an LC 50 of 0.1 mg mL -1 . The in vivo assay on the zebrafish embryos showed a linear relationship between the polysaccharide concentration and growth retardation at 7.8 hpf mL mg -1 , with an LC 50 of about 5.2 mg mL -1 at 48 hpf. At concentrations near the LC 50 , toxic effects, such as pericardial edema or chorion lysis, could be found in the experimental larvae. Our in vitro study supports the potential use of polysaccharides extracted from U. rigida as candidates for treating human colon cancer. However, the in vivo assay on zebrafish indicated that the potential use of ulvan as a promising, safe compound should be limited to specific concentrations below 0.001 mg mL -1 since it revealed side effects on the embryonic growth rate and osmolar balance.
Keyphrases
- endothelial cells
- induced pluripotent stem cells
- pluripotent stem cells
- climate change
- mass spectrometry
- high resolution
- high throughput
- young adults
- simultaneous determination
- water soluble
- anti inflammatory
- genetic diversity
- smoking cessation
- working memory
- single cell
- single molecule
- lymph node metastasis
- neural network
- aedes aegypti